The Hektros Hi5i cell-culture wells were developed to perfectly fit the Hi5 live-cell-imaging incubator and provide optimal cultivation conditions as well as best optical properties for the use with various microscopy techniques.

The Hi5i standard wells are completely made of highly transparent and plasma treated polystyrene to be as comparable as possible to conventional cell-culture wells e.g. petri-dishes or 6- and 12-well-plates in terms of material and growth surface.

The Hi5i glass-bottom wells are made of polystyrene walls an a borosilicate glass bottom. The glass bottoms with a thickness of 160µm provide best optical properties and are suitable for high resolution microscopy techniques and magnifications up to 100 fold. The Hi5i glass-bottom wells are produced without additional chemicals like glue through integration into the injection molding process. To meet the needs of various microscopy techniques the Hi5i glass-bottom wells are available fully transparent or with white walls especially for luminescence and with black walls for fluorescence imaging.

All Hi5i wells are packed sterile and seated within a polystyrene carrier. They are protected by a HiCult cell-culture dish and can therefore be easily pre-cultivated in a standard CO2-incubator without the need to setup the Hi5 incubator. This feature makes the Hi5i-wells not only the perfect insert for the Hi5 incubator, but also convenient and high quality cell-culture wells for general microscopy applications.